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PreDictor AIEX screening plate 20 μl contains 20 μl per well of the corresponding media. PreDictor AIEX screening plate 2μl/6μl contains following chromatography media volumes per well: Capto Q 2 μl, Capto DEAE 2 μl, Q Sepharose Fast Flow 6 μl and Capto adhere 6 μl. PreDictor Capto AIEX polishing screening plate 20 μl contains 20 μl per well of the corresponding media. PreDictor Capto AIEX polishing screening plates 2μl/6μl contains following chromatography media volumes per well: Capto Q 2 μl, Capto Q ImpRes 6 μl, Capto adhere 6 μl and Capto adhere ImpRes 6 ul. PreDictor CIEX screening plate 20 μl contains 20 μl per well of the corresponding media. PreDictor CIEX screening plates 2μl/6μl contains following chromatography media volumes per well: Capto S 2 μl, SP Sepharose Fast Flow 6 μl and Capto MMC 6 μl. PreDictor Capto CIEX polishing screening plate 20 μl contains 20 μl per well of the corresponding media. PreDictor Capto CIEX polishing screening plates 2μl/6μl contains following chromatography media volumes per well: Capto S ImpAct 2 μl, Capto SP ImpRes 6 μl and Capto MMC ImpRes 6 μl. For total medium suspension volume per well, see Table 4.2. Note that the total medium suspension volume per well is larger than the chromatography medium volume. Isotherm plates PreDictor Adsoption isotherm plates 8ĭifferent media volume in different wells (2 μl, 4 μl, 6 μl, 8 μl, 20 μl and 50 μl) Screening plates PreDictor Capto CIEX polishing screening 2 PreDictor CIEX screening plate 3 PreDictor Capto AIEX polishing screening 4 PreDictor AIEX screening plate 5 PreDictor HIC screening high hydrophobicity 6 PreDictor HIC screening low hydrophobicity 7Ģ μl/6 μl or 20 μl 2 μl/6 μl or 20 μl 2 μl/6 μl or 20 μl 6 μl or 50 μl 6 μl or 50 μl Hydrophobic interaction chromatography media PreDictor Capto Butyl PreDictor Butyl Sepharose 4 Fast Flow PreDictor Butyl-S Sepharose 6 Fast Flow PreDictor Capto Octyl PreDictor Octyl Sepharose 4 Fast Flow PreDictor Capto Phenyl (high sub) PreDictor Phenyl Sepharose 6 Fast Flow (high sub) PreDictor Phenyl Sepharose 6 Fast Flow (low sub)Ħ μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl 6 μl or 50 μl Multimodal chromatography media PreDictor Capto MMC PreDictor Capto MMC ImpRes PreDictor Capto adhere PreDictor Capto adhere ImpResĦ μl, 20 μl or 50 μl 6 μl or 20 μl 6 μl, 20 μl or 50 μl 6 μl or 20 μlĪffinity chromatography media PreDictor MabSelect™ PreDictor MabSelect SuRe™ PreDictor MabSelect SuRe LX PreDictor MabSelect Xtra™ PreDictor Capto LĦ μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μl Single media plates Cation exchange chromatography media PreDictor Capto™ S ImpAct PreDictor Capto SP ImpRes PreDictor Capto S PreDictor SP Sepharose™ Fast FlowĢ μl or 20 μl 6 μl or 20 μl 2 μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μlĪnion exchange chromatography media PreDictor Capto Q ImpRes PreDictor Capto Q PreDictor Capto DEAE PreDictor Q Sepharose Fast FlowĦ μl or 20 μl 2 μl, 20 μl or 50 μl 2 μl, 20 μl or 50 μl 6 μl, 20 μl or 50 μl Table 1.1: Available PreDictor plate products Product This means that once the experimental subspace has been found, optimization and scale-up can easily be done on columns using ÄKTA™ systems. All chromatography media used in PreDictor plates are available in prepacked columns, such as HiTrap™ and HiScreen™ columns, and in bulk packs. This allows screening of a large experimental space to identify the subspace that is the most relevant with respect to one or several defined responses. As a result of parallel screening of chromatographic conditions, a large number of experimental conditions may be evaluated simultaneously. The choice of PreDictor plate depends on the type of application, see Table 3.1, Table 3.2 and Chapter 3 PreDictor plate selection, on page 9 for details. Each well in a PreDictor plate is prefilled with a defined amount of chromatography medium. They can be used in automated workflows using robotic systems, or can be operated manually using multi-channel pipettes. PreDictor plates support high-throughput process development (HTPD) by allowing parallel screening of chromatographic conditions. Introduction PreDictor plate are disposable 96-well filter plates prefilled with GE Healthcare Life Sciences BioProcess™ chromatography media (resins), see Table 1.1.
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PreDictor plates Instructions 28-9258-34 AK
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Safety For use and handling of the products in a safe way, please refer to the Safety Data Sheets. Intended use The products are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. Please read these instructions carefully before using the products. Hp filter lambda for annual data.Introduction.